Recombinant ACTA2 Antibody

This recombinant Alpha Smooth Muscle Actin / ACTA2 antibody reacts to human and mouse alpha smooth muscle Actin (alpha-Actin-2) . It may also react to bovine or rat alpha smooth muscle Actin, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:2500 (1), Western blot: 1:1000-1:2000

UniProt

P62736

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A peptide corresponding to the N-terminus of human alpha smooth muscle Actin was used as the immunogen for this recombinant ACTA2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM253

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant ACTA2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant ACTA2 antibody is available for research use only.

Storage Conditions

Store the recombinant ACTA2 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.