Recombinant phospho-AKT Antibody (Ser473)

This recombinant phospho-AKT antibody reacts to AKT1/2/3 only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. It may also react to bovine, mouse or rat phospho-AKT (Ser473), as predicted by immunogen homology

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:200-1:500 (1), Western blot: 1:1000-1:2000

UniProt

P31749, P31751, Q9Y243

Host

Rabbit

Reactivity

Human

Immunogen

A phospho-peptide corresponding to human phospho-Akt (Ser473) was used as the immunogen for this recombinant phospho-AKT antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM251

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant phospho-AKT antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant phospho-AKT antibody is available for research use only.

Storage Conditions

Store the recombinant phospho-AKT antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Prediction Reactivity

Bovine, Mouse, Rat

Image Legend

Western blot test of lysates from 293 cells grown in medium with serum, serum starved, or insulin treated, using recombinant phospho-AKT antibody at 1:1000.