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Recombinant Caspase-3 Antibody

This recombinant Caspase-3 antibody reacts to human full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:2500 (1), Western blot: 1:1000-1:2000

UniProt

P42574

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to residues within human Caspase-3 subunit p17 was used as the immunogen for this recombinant Caspase-3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM250

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Caspase-3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Caspase-3 antibody is available for research use only.

Storage Conditions

Store the recombinant Caspase-3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of Jurkat cell lysate, untreated or treated with Etoposide, using recombinant Caspase-3 antibody at 1:1000.

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