Recombinant CD146 Antibody

This recombinant CD146 antibody reacts to human CD146 (Cell surface glycoprotein MUC18) . It may also react to the mouse and rat protein, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:200-1:500 (1), Western blot: 1:1000-1:2000

UniProt

P43121

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the residues near C-terminus of human CD146 was used as the immunogen for this recombinant CD146 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM249

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant CD146 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant CD146 antibody is available for research use only.

Storage Conditions

Store the recombinant CD146 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CD146 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Prediction Reactivity

Mouse, Rat

Image Legend

Western blot testing of human A375 cell lysate with recombinant CD146 antibody at 1:1000. Observed molecular weight 70-120 kDa depending on glycosylation level.