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Recombinant CD31 Antibody

This recombinant CD31 antibody reacts to human CD31/PECAM-1.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:2500 (1), Western blot: 1:1000-1:2000

UniProt

P16284

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding from the cytoplasmic domain of human CD31 was used as the immunogen for this recombinant CD31 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM247

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant CD31 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant CD31 antibody is available for research use only.

Storage Conditions

Store the recombinant CD31 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CD31 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

Western blot testing of human Jurkat cell lysate with recombinant CD31 antibody at 1:1000. Expected molecular weight: 83-130 kDa depending on level of glycosylation.

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