Recombinant E-Cadherin Antibody

This recombinant E-Cadherin antibody reacts to human E-cadherin/Cadherin-/CDH1.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry: 1:500-1:1000 (1), Western blot: 1:1000-1:2000

UniProt

P12830

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to E-Cadherin/CDH1 was used as the immunogen for this recombinant E-Cadherin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM244

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant E-Cadherin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant E-Cadherin antibody is available for research use only.

Storage Conditions

Store the recombinant E-Cadherin antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Location

Membrane, cytoplasmic

Image Legend

Western blot of human MCF7 lysate using recombinant E-Cadherin antibody at 1:1000. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on glycosylation level) .