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Recombinant HIF1A Antibody

This recombinant HIF1A antibody reacts to human Hypoxia-inducible factor 1-alpha.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000 (1), Western blot: 1:1000-1:2000

UniProt

Q16665

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to Hypoxia-inducible factor 1-alpha was used as the immunogen for this recombinant HIF1A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM242

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant HIF1A antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant HIF1A antibody is available for research use only.

Storage Conditions

Store the recombinant HIF1A antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Location

Nuclear, possible cytoplasmic

Image Legend

Western blot test of Jurkat cell lysate, untreated or treated with Cobalt Chloride (CoCl2), using recombinant HIF1A antibody at 1:1000. Routinely observed molecular weight: 100~120 kDa.

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