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Recombinant Cyclin D1 Antibody

This recombinant Cyclin D1 antibody reacts to human Cyclin D1. It may also react to the bovine, mouse or rat protein, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000 (1), Western blot: 1:1000-1:2000

UniProt

P24385

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to Cyclin D1 was used as the immunogen for this recombinant Cyclin D1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM241

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Cyclin D1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Cyclin D1 antibody is available for research use only.

Storage Conditions

Store the recombinant Cyclin D1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Prediction Reactivity

Bovine, Mouse, Rat

Location

Nuclear

Image Legend

Western blot testing of human HeLa cell lysate with recombinant Cyclin D1 antibody at 1:1000. Predicted molecular weight: 32-36 kDa.

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