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Recombinant HER2 Antibody

This recombinant HER2 antibody reacts to Human c-erbB-2/HER-2. It may also react to mouse or rat c-erbB-2/HER-2, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:100-1:400 (1), Western blot: 1:1000

UniProt

P04626

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human c-erbB2/HER2 was used as the immunogen for this recombinant HER2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM228

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant HER2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant HER2 antibody is available for research use only.

Storage Conditions

Store the recombinant HER2 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HER2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Location

Cell surface, cytoplasm, nucleus

Image Legend

Western blot of MCF7, PC3, Jurkat, and A375 cell lysate using recombinant HER2 antibody. Only the breast cancer line MCF7 showed a response.

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