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Recombinant His Tag Antibody

This recombinant His Tag antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.1-1 µg/mL, Immunoprecipitation: 0.5-2ug/mg, Immunocytochemistry: 0.5-2 µg/mL, Flow cytometry: 0.5-2 µg/mL, Immunohistochemistry: 0.1-1 µg/mL (1)

Host

Rabbit

Reactivity

All Species Expected

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this recombinant His Tag antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM146

Type

Recombinant

Applications

WB, IP, ICC, FACS , IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant His Tag antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant His Tag antibody is available for research use only.

Storage Conditions

Store the recombinant His Tag antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

IP and detect of 293T cells expressing a His-Tag fusion protein using recombinant His Tag antibody. (1) Whole lysate control, (2) IP by rabbit IgG control; (3) IP by RM146 mAb.

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