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Recombinant Histone H3.3 Antibody

This recombinant Histone H3.3 antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, ELISA: 0.2-1 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A peptide corresponding to human Histone H3.3 was used as the immunogen for this recombinant Histone H3.3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM190

Type

Recombinant

Applications

WB, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Histone H3.3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Histone H3.3 antibody is available for research use only.

Storage Conditions

Store the recombinant Histone H3.3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3.3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using recombinant Histone H3.3 antibody at 1 ug/ml.

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