Recombinant Histone H3 Antibody

This recombinant Histone H3 antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other Histone proteins.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.01-0.25 µg/mL, Immunocytochemistry: 1 µg/mL-5 µg/mL, ELISA: 0.2-1 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A peptide corresponding to the C-terminus of human Histone H3 was used as the immunogen for this recombinant Histone H3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM188

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Histone H3 antibody is available for research use only.

Storage Conditions

Store the recombinant Histone H3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of recombinant Histone H3.3 and H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using recombinant Histone H3 antibody at 0.025 µg/ml.