Recombinant Histone H2AX Antibody / H2AFX

This recombinant Histone H2AX antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, Immunocytochemistry: 1-2 µg/mL, ELISA: 0.2-1 µg/mL

UniProt

P16104

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A peptide corresponding to the C-terminus of human Histone H2AX was used as the immunogen for this recombinant Histone H2AX antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM214

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Histone H2AX antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Histone H2AX antibody is available for research use only.

Storage Conditions

Store the recombinant Histone H2AX antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using recombinant Histone H2AX antibody at 0.5 ug/ml. Predicted molecular weight ~15 kDa.