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Recombinant MacroH2A.1 Antibody / H2AFY

This recombinant MacroH2A.1 antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, Immunocytochemistry: 1-2 µg/mL, ELISA: 0.2-1 µg/mL

UniProt

Q9QZQ8

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone macroH2A1 was used as the immunogen for this recombinant MacroH2A.1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM248

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant MacroH2A.1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant MacroH2A.1 antibody is available for research use only.

Storage Conditions

Store the recombinant MacroH2A.1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant MacroH2A.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of acid extracts from K562 cells, using recombinant MacroH2A.1 antibody at 1 ug/ml. Predicted molecular weight ~40 kDa.

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