Recombinant 5hmC Antibody

This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Product Specifications

CAS Number

9000-83-3

Specifications

Dot blot: 0.2-1 µg/mL, ELISA: 0.1-1 µg/mL, Immunohistochemistry: 0.1-1 µg/mL (1), Immunocytochemistry: 0.5-2 µg/mL, hMeDIP: 0.2 µg/mL- 2 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM236

Type

Recombinant

Applications

DB, hMeDIP, ICC, IHC, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant 5hmC antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant 5hmC antibody is available for research use only.

Storage Conditions

Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

ELISA of single stranded DNA using recombinant 5hmC antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5mC) DNA, and 5-Hydroxymethylcytosine (5hmC) DNA. A serial dilution of RM236 mAb was used as the primary, and an anti-rabbit IgG AKP secondary.