Recombinant 5mC Antibody

This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

Product Specifications

CAS Number

9000-83-3

Specifications

Dot blot: 0.5-2 µg/mL, ELISA: 0.1-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL, MeDIP: 0.2 µg/mL- 2 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

BSA-conjugated 5-methylcytosine was used as the immunogen for this recombinant 5mC antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM231

Type

Recombinant

Applications

DB, MeDIP, ICC, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant 5mC antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant 5mC antibody is available for research use only.

Storage Conditions

Store the recombinant 5mC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant 5mC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

ELISA of single stranded DNA using recombinant 5mC antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary, and an anti-rabbit IgG AKP secondary.