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Recombinant Gamma H2AX Antibody (phospho S139)

This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, ELISA: 0.2-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL

UniProt

P16104

Host

Rabbit

Reactivity

Human

Immunogen

A phospho-peptide corresponding to phospho-Histone H2AX (S139) was used as the immunogen for this recombinant Gamma H2AX antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM224

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Gamma H2AX antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Gamma H2AX antibody is available for research use only.

Storage Conditions

Store the recombinant Gamma H2AX antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Gamma H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of acid extracts of HeLa cells treated or non-treated with UV, using recombinant Gamma H2AX antibody at 0.5 ug/ml, showed a band of Histone H2A.X phosphorylated at Serine 139 in HeLa cells.

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