Recombinant phospho-Histone H2B Antibody (Ser14)

This recombinant phospho-Histone H2B antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, Immunocytochemistry: 1-2 µg/mL, ELISA: 0.2-1 µg/mL

UniProt

P33778

Host

Rabbit

Reactivity

Human

Immunogen

A phospho-peptide corresponding to phospho-Histone H2B (Ser14) was used as the immunogen for this recombinant phospho-Histone H2B antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM238

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant phospho-Histone H2B antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant phospho-Histone H2B antibody is available for research use only.

Storage Conditions

Store the recombinant phospho-Histone H2B antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H2B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot test of acid extracts of HeLa cells treated or non-treated with Nocodazole, using recombinant phospho-Histone H2B antibody at 0.5 ug/ml, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.