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Recombinant H2BK20ac Antibody

This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac) . No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, ELISA: 0.2-1 µg/mL, Immunocytochemistry: 1-2 µg/mL

UniProt

P33778

Host

Rabbit

Reactivity

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20) was used as the immunogen for this recombinant H2BK20ac antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM235

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H2BK20ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant H2BK20ac antibody is available for research use only.

Storage Conditions

Store the recombinant H2BK20ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H2BK20ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2BK20ac antibody at 0.5 ug/ml.

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