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Recombinant Histone H3 Antibody

This recombinant Histone H3 antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The recombinant Histone H3 antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, ELISA: 0.5-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL

UniProt

Q16695

Host

Rabbit

Reactivity

All Species Expected

Immunogen

An unmodified peptide corresponding to the N-terminus of Histone H3 was used as the immunogen for this recombinant Histone H3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM186

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Histone H3 antibody is available for research use only.

Storage Conditions

Store the recombinant Histone H3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot test of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated and untreated with Nocodazole, and recombinant Histone H3.1 protein, using recombinant Histone H3 antibody at 1 ug/ml, showed a band of Histone H3 with unmodified Lys4.

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