Recombinant H4K8ac Antibody

This recombinant H4K8ac antibody reacts to Histone H4 acetylated at Lysine 8 (K8ac) . No cross reactivity with other acetylated Lysines in Histone H4.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, ELISA: 0.2-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL

UniProt

P62805

Host

Rabbit

Reactivity

All Species Expected

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys8) was used as the immunogen for this recombinant H4K8ac antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM201

Type

Recombinant

Applications

WB, ELISA, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H4K8ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant H4K8ac antibody is available for research use only.

Storage Conditions

Store the recombinant H4K8ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H4K8ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4 using recombinant H4K8ac antibody at 0.5 ug/ml showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.