Recombinant H3K79me2 Antibody

This recombinant H3K79me2 antibody reacts to Histone H3 dimethylated at Lysine 79 (K79me2) . No cross reactivity with monomethylated (K79me1) or trimethylated Lysine 79 (K79me3), or other methylation in Histone H3.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.25-1 µg/mL, Immunohistochemistry: 0.1-1 µg/mL (1), ChIP: 2-10ug/mg, ELISA: 0.2-1 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM181

Type

Recombinant

Applications

WB, ELISA, ChIP, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H3K79me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant H3K79me2 antibody is available for research use only.

Storage Conditions

Store the recombinant H3K79me2 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K79me2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me2 antibody at 0.25 ug/ml showed a band of Histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.