Recombinant H3K27me3 Antibody

This recombinant H3K27me3 antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3) . No cross reactivity with monomethylated (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-2.5 µg/mL, ELISA: 0.5-1 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

Human

Immunogen

A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys27) was used as the immunogen for this recombinant H3K27me3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM175

Type

Recombinant

Applications

WB, ELISA, IHC-P

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H3K27me3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant H3K27me3 antibody is available for research use only.

Storage Conditions

Store the recombinant H3K27me3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K27me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Human

Location

Nuclear

Image Legend

Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K27me3 antibody at 1 ug/ml showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells.