Recombinant H3K9me2 Antibody

This recombinant H3K9me2 antibody reacts to Histone H3 dimethylated at Lysine 9 (K9me2) . No cross reactivity with monomethylated (K9me1) or trimethylated Lysine 9 (K9me3), or other methylation in Histone H3.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.25-1 µg/mL, ChIP: 2-10ug/mg of lysate, Immunocytochemistry: 0.5-2 µg/mL, ELISA: 0.2-1 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9) was used as the immunogen for this recombinant H3K9me2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM151

Type

Recombinant

Applications

WB, ELISA, ChIP, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H3K9me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant H3K9me2 antibody is available for research use only.

Storage Conditions

Store the recombinant H3K9me2 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K9me2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K9me2 antibody at 0.5 ug/ml showed a band of Histone H3 dimethylated at Lysine 9 (K9me2) in HeLa cells.