Recombinant H3K9ac Antibody

This recombinant H3K9ac antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac) . No cross reactivity with other acetylated Lysines in histone H3.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.25-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL, ChIP: 2 µg/mL-10ug/mg of lysate, Immunohistochemistry: 0.1-1 µg/mL (1), ELISA: 0.5-1 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

All Species Expected

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys9) was used as the immunogen for this recombinant H3K9ac antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM161

Type

Recombinant

Applications

WB, ELISA, ChIP, ICC, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H3K9ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant H3K9ac antibody is available for research use only.

Storage Conditions

Store the recombinant H3K9ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K9ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K9ac antibody at 0.25 ug/ml showed a band of Histone H3 acetylated at Lysine 9 in treated HeLa cells.