Recombinant H3K14ac Antibody

This recombinant H3K14ac antibody reacts to Histone H3 acetylated at Lysine 14 (K14ac), which is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), 9 (K9ac), 18 (K18ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), or 79 (K79ac) in histone H3.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-2 µg/mL, Immunocytochemistry: 0.5-2 µg/mL, ChIP: 2-10ug/mg of lysate, ELISA: 0.2-1 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

All Species Expected

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) was used as the immunogen for this recombinant H3K14ac antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM130

Type

Recombinant

Applications

WB, ELISA, ChIP, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H3K14ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant H3K14ac antibody is available for research use only.

Storage Conditions

Store the recombinant H3K14ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K14ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western Blot of acid extracts from human HeLa cells untreated (-) or treated (+) with sodium butyrate using the recombinant H3K14ac antibody at 0.5 ug/ml.