Recombinant GAPDH Antibody (Loading Control)

This recombinant GAPDH antibody reacts to Glyceraldehyde 3-phosphate dehydrogenase.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000, Immunoprecipitation: 1:200, Chromatin IP (ChIP) : 1:200, Immunocytochemistry: 1:200, Immunohistochemistry: 1:200 (1)

UniProt

P04406

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A peptide corresponding to the C-terminus of GAPDH was used as the immunogen for this recombinant GAPDH antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM114

Type

Recombinant

Applications

WB, IP, ICC, IHC, ChIP

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant GAPDH antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant GAPDH antibody is available for research use only.

Storage Conditions

Store the recombinant GAPDH antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.