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Recombinant Beta Actin Antibody (Loading Control)

This recombinant Beta Actin antibody reacts to b-Actin (ACTB/Actin, cytoplasmic 1) . No cross reactivity with other isoforms of Actin.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000, Immunoprecipitation: 1:200, Chromatin IP (ChIP) : 1:200, Immunocytochemistry: 1:200, Immunohistochemistry: 1:200-1:1000

UniProt

P60709

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A peptide corresponding to the N-terminus of b-Actin was used as the immunogen for this recombinant Beta Actin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM112

Type

Recombinant

Applications

WB, IP, ICC, IHC, ChIP

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Beta Actin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Beta Actin antibody is available for research use only.

Storage Conditions

Store the recombinant Beta Actin antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Beta Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of human A431 cells using the recombinant Beta Actin antibody at a 1:1000. Predicted molecular weight ~42 kDa.

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