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Recombinant Alpha Tubulin Antibody / TUBA1B (Loading Control)

This recombinant Alpha Tubulin antibody reacts to a-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000, Immunofluorescence/Immunocytochemistry: 1:200

UniProt

P68363

Host

Rabbit

Reactivity

All Species Expected

Immunogen

A peptide corresponding to the C-terminus of a-Tubulin was used as the immunogen for this recombinant Alpha Tubulin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM113

Type

Recombinant

Applications

WB, IF/ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Alpha Tubulin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Alpha Tubulin antibody is available for research use only.

Storage Conditions

Store the recombinant Alpha Tubulin antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Alpha Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

Western blot of human A431 cells with recombinant Alpha Tubulin antibody at 1:1000. Predicted molecular weight ~50 kDa.

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