Recombinant Phosphotyrosine Antibody

This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000 -1:5000, Immunoprecipitation: 1:200-1:1000, Chromatin IP (ChIP) : 1:200-1:1000, Immunocytochemistry: 1:200-1:500, Immunohistochemistry: 1:200-1:500 (1)

Host

Rabbit

Reactivity

All Species Expected

Immunogen

Phosphotyrosine-BSA conjugate was used as the immunogen for this recombinant Phosphotyrosine antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM111

Type

Recombinant

Applications

WB, IP, ICC, IHC, ChIP

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Phosphotyrosine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Phosphotyrosine antibody is available for research use only.

Storage Conditions

Store the recombinant Phosphotyrosine antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

ICC test of serum-starved human A431 cells non-treated or treated with EGF, using the recombinant Phosphotyrosine antibody at 1:500 (red) and DAPI (blue) .