Recombinant Phosphothreonine Antibody

This recombinant Phosphothreonine antibody reacts with threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500 -1:2000 dilution, Immunoprecipitation: 1:100-1:500 dilution, Chromatin IP (ChIP) : 1:100-1:500 dilution, Immunocytochemistry: 1:100-1:500 dilution, Immunohistochemistry: 1:100-1:500 dilution (1)

Host

Rabbit

Reactivity

All Species Expected

Immunogen

Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide was used as the immunogen for this recombinant Phosphothreonine antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM102

Type

Recombinant

Applications

WB, IP, ICC, IHC, ChIP

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Phosphothreonine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Phosphothreonine antibody is available for research use only.

Storage Conditions

Store the recombinant Phosphothreonine antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Phosphothreonine antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

ICC of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:500 (PE-secondary, red) and DAPI (blue) .