Recombinant Human IgG4 Antibody / Hinge region

This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 50ng/well-200ng/well (Capture) ; 0.05-0.2 µg/mL (Detection), Immunocytochemistry: 1-10 µg/mL, Immunohistochemistry: 1-10 µg/mL (1)

UniProt

P01861

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM120

Type

Recombinant

Applications

ICC, IHC, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Human IgG4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Human IgG4 antibody is available for research use only.

Storage Conditions

Store the recombinant Human IgG4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.