Recombinant Human IgE Antibody (Biotin Conjugate)

This recombinant Human IgE antibody reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 10ng/well-100ng/well (Capture) ; 0.01-0.1 µg/mL (Detection)

UniProt

P01854

Host

Rabbit

Reactivity

Human

Immunogen

Human IgE was used as the immunogen for this recombinant Human IgE antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM122

Type

Recombinant

Applications

ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Biotin Conjugate

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Human IgE antibody at -20oC.

Limitations

This recombinant Human IgE antibody is available for research use only.

Storage Conditions

Store the recombinant Human IgE antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgE antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Image Legend

ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgElambda from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.