Recombinant Human IgG Antibody

This recombinant Human IgG antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including G1, G2, G3, and G4. No cross reactivity with other human heavy chains or mouse/rat/goat IgG.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 50ng/well-200ng/well (Capture) ; 0.05-0.2 µg/mL (Detection), Immunocytochemistry: 0.5-2 µg/mL, Immunohistochemistry: 0.5-2 µg/mL (1)

UniProt

P01857, P01859, P01860, P01861

Host

Rabbit

Reactivity

Human

Immunogen

Human IgG was used as the immunogen for this recombinant Human IgG antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM116

Type

Recombinant

Applications

ICC, IHC, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Human IgG antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Human IgG antibody is available for research use only.

Storage Conditions

Store the recombinant Human IgG antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.