Recombinant Mouse IgG2b Antibody

This recombinant Mouse IgG2b antibody reacts to the Fc region of mIgG2b. No cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human/rat/goat IgG.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 0.005 µg/mL-0.2 µg/mL, Western Blot (stronger on non-reduced samples) : 0.1-0.5 µg/mL

UniProt

P01867

Host

Rabbit

Reactivity

Mouse

Immunogen

Mouse IgG2b was used as the immunogen for this recombinant Mouse IgG2b antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM108

Type

Recombinant

Applications

WB, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Mouse IgG2b antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Mouse IgG2b antibody is available for research use only.

Storage Conditions

Store the recombinant Mouse IgG2b antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of nonreduced (-) and reduced (+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa) .