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Recombinant Mouse IgG1 Antibody

This recombinant Mouse IgG1 antibody reacts to the Fc region of mouse IgG1. No cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mIgG2b, and may also react to goat IgG.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 0.005-0.2 µg/mL, Western Blot: 0.1-0.5 µg/mL

UniProt

P01868

Host

Rabbit

Reactivity

Mouse

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse IgG1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM106

Type

Recombinant

Applications

WB, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Mouse IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Mouse IgG1 antibody is available for research use only.

Storage Conditions

Store the recombinant Mouse IgG1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of nonreduced (-) and reduced (+) mouse IgG1 (20ng/lane), using 0.2ug/mL of recombinant Mouse IgG1 antibody. This mAb reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa) .

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