ATG4B Antibody
Autophagy is the cellular mechanism of degradation and turnover of unnecessary or dysfunctional cellular components. The unneeded components are engulfed in vesicles called autophagosomes, where they are broken down and recycled. The ‘autophagy-related gene’ ATG4B is a regulator of autophagasome maturation. The microtubule-associated protein LC3-I is lipidated (called LC3-II in its lipidated state) and recruited to the autophagosomal membrane where it is of vital importance in the maturation of autophagosomes. ATG4B regulates LC3-II availability by modulating LC3-I lipidation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 4-6 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant protein fragment from the N-terminal region of ATG4B was used as the immunogen for this antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the ATFG4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATG4B antibody is available for research use only.
Storage Conditions
Aliquot the ATFG4B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, ATG4B antibody titration may be required for optimal results.
Image Legend
Western blot testing of human samples with ATG4B antibody at 4ug/ml.
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