S100A8 Antibody
The S100 family of proteins are found in vertebrates and are characterized by two calcium-binding sites that have a helix-loop-helix conformation. The family of 24 is divided into three subgroups based on function: those with intracellular, intracellular plus extracellular, and extracellular regulatory effects. The calcium-binding protein S100A8, also called MRP8 or Myeloid-related protein 8, and Calgranulin A, forms a heterodimer with S100A9, called Calprotectin. The heterodimer is expressed by granulocytes, monocytes, and early differentiation states of macrophages, and is known to be an important pro-inflammatory mediator in acute and chronic inflammation. S100A8 and 9 are DAMPs, or Damage-associated molecular pattern molecules, and are able to activate receptors of the innate immune system, one being TLR4.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
A recombinant protein fragment from the N-terminal region of human S100A8 was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SA6279
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the S100A8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This S100A8 antibody is available for research use only.
Storage Conditions
Aliquot the S100A8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, S100A8 antibody titration may be required for optimal results.
Image Legend
Western blot testing of human samples with S100A8 antibody (clone SA6279) at 2ug/ml. Predicted molecular weight ~11 kDa.
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