ATF6 Antibody-
ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response) . Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL (2), Immunohistochemistry (FFPE) : 5 µg/mL (1)
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AF6229
Applications
WB, IHC-P
Purity
Protein G purified monoclonal antibody
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATF6 antibody is available for research use only.
Storage Conditions
Aliquot the antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of human samples with ATF6 antibody (AF6229) at 2ug/ml. Predicted molecular weight: 75~90kDa.
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