ICAD Antibody
During apoptosis, toxic or useless cells are broken down for removal. DFF, or DNA fragmentation factor, is a heterodimeric protein which mediates DNA fragmentation and chromatin condensation during apoptosis. DFFB is the active DFF subunit but remains inactive while bound to DFFA, also call ICAD (Inhibitor of caspase-activated DNase) . Caspase-3 cleaves ICAD, which then disassociates from DFFB. The disassociation removes the inhibitory effects of ICAD, activating DFFB and beginning the DNA fragmentation process.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 4-8 µg/mL
Host
Rabbit
Reactivity
Mouse
Immunogen
A recombinant protein fragment from human ICAD was used as the immunogen for this antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ICAD antibody is available for research use only.
Storage Conditions
Aliquot the antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Image Legend
Western blot test of mouse samples with ICAD antibody at 4ug/ml.
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