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Acid Phosphatase / ACP1 Antibody

Acid phosphatases (AP) are a broad family of enzymes that differ widely in their AA length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-3 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant protein fragment from the N-terminal portion of Acid Phosphatase / ACP1 was used as the immunogen for this antibody. This AA sequence is common to the A, C and D isoforms.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Aliquot the ACP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Acid Phosphatase / ACP1 antibody is available for research use only.

Storage Conditions

Aliquot the ACP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Provided assay concentrations are suggestions only, ACP1 antibody titration may be required for optimal results. </p>1. This antibody will detect the A, C and D isoforms of Acid Phosphatase.</p>

Image Legend

Western blot testing of human samples with Acid Phosphatase / ACP1 antibody at 1ug/ml.

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