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Acid Phosphatase Antibody (Isoform 2 specific)

Acid phosphatases are a broad family of enzymes that differ widely in their amino acid length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid Phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-3 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

The amino acid sequence used as immunogen for this antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Aliquot the Acid Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Acid Phosphatase antibody is available for research use only.

Storage Conditions

Aliquot the Acid Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Provided assay concentrations are suggestions only, Acid Phosphatase antibody titration may be required for optimal results. </p>1. This antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase.</p>

Image Legend

Western blot testing of human samples using Acid Phosphatase antibody at 1ug/ml.

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