GAPDH Antibody Loading Control
Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 3-5 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog
Immunogen
A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GAP259
Applications
WB, IHC-P
Purity
Protein G Affinity Chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot and store the loading control antibody frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Limitations
This GAPDH antibody loading control is available for research use only.
Storage Conditions
Aliquot and store the loading control antibody frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.
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