SIN1 Antibody / SAPK interacting protein 1 / MAPKAP1
MTOR, or Mammalian target of rapamycin, a large protein kinase, is a regulator of cell growth and survival via its mTORC1 (mTOR + GBL + Raptor) and mTORC2 (mTOR + GBL + SIN1 + Rictor) complexes. SIN1 is an essential component of mTORC2, which activates Akt by phosphorylating Ser473. Phosphorylation of SIN1 at Thr86/398 causes its dissociation from the mTORC2 complex, inhibiting mTORC2s kinase activity.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-3 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
A recombinant protein fragment from the C-terminal region of human SIN1 was used as the immunogen for this antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Protein A purified antibody
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Limitations
This SIN1 antibody is available for research use only.
Storage Conditions
Aliquot and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, SIN1 antibody titration may be required for optimal results.
Image Legend
Western blot testing of human samples with SIN1 antibody at 1ug/ml.
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