Caspase-3 Antibody, Pro and Active
Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12) . Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 5 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
The large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CAS3836
Applications
WB, IHC-P
Purity
Protein G purified monoclonal antibody
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the Pro and Active Caspase-3 antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Limitations
This Caspase-3 antibody is available for research use only.
Storage Conditions
Aliquot the Pro and Active Caspase-3 antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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