Beta Tubulin Antibody Loading Control
There are five mammalian members of the tubulin family: alpha, beta, gamma, delta, and epsilon. Heterodimers of alpha and beta tubulin make up microtubules, which along with microfilaments and intermediate filaments, make up the cytoskeleton. Due to its ubiquitous presence in mammalian cells, it is often used a loading control protein in western blot. An antibody to beta-tubulin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
A recombinant protein fragment from the C-terminal region of human beta tubulin was used as the immunogen for this antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Protein A Affinity Chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Limitations
This beta Tubulin antibody loading control is available for research use only.
Storage Conditions
Aliquot the loading control antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Image Legend
Western blot testing of human samples with beta Tubulin antibody at 2ug/ml. Predicted molecular weight: 50-55 kDa.
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