Beta Actin Antibody Loading Control
Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-3 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Rabbit, Chicken, Dog
Immunogen
A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Protein A Affinity Chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Limitations
This beta Actin antibody loading control is available for research use only.
Storage Conditions
Aliquot the loading control antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.
Image Legend
Western blot testing of human samples with beta Actin antibody at 1 ug/ml.
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