IKKb Antibody
NF-kB is sequestered in the cytoplasm by IkBa, unable to translocate to the nucleus. IkB is part of the canonical pathway for NF-kB activation. IKKb is part of the IkB kinase complex, along with a second catalytic subunit, IKKa, and a regulatory subunit, IKKg. Along with IKKa, IKKb phosphorylates IkBa leading to its degradation and release of inhibition of NF-kB. The RELA subunit of active NF-kB is then free to enter the nucleus and initiate a number of signaling cascades.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A recombinant protein fragment of IKKb was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
IKB355
Applications
WB
Purity
Protein G purified antibody
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the IKKb antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IKKb antibody is available for research use only.
Storage Conditions
Aliquot the IKKb antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, IKKb antibody titration may be required for optimal results.
Image Legend
Western blot testing of human samples with IKKb antibody IKB355 at 2ug/ml.
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