Rabbit Isotype Control Polyclonal Antibody

Isotype control antibodies are negative controls used to measure non-specific binding or background levels of a primary antibody in different cell types. They are not specific to any target in a cell, yet retain all non-specific characteristics of the antibodies they control.

Product Specifications

CAS Number

9007-83-4

Specifications

FACS: assay dependent, IHC: assay dependent, IF: assay dependent

Host

Rabbit

Immunogen

This is purified naive rabbit sera and as such there was no immunogen.

Clonality

Polyclonal

Isotype

IgG

Applications

FACS, IHC, IF

Purity

Protein A purified IgG fraction

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Aliquot the rabbit antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

Limitations

This Rabbit isotype control antibody is available for research use only.

Storage Conditions

Aliquot the rabbit antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.

Formulation

0.5 mg/mL antibody in PBS with 0.05% sodium azide

Applications Notes

Generally to be used at the same concentration as the sample being controlled for.

Image Legend

FACS testing of Rabbit IgG isotype control antibody on human samples. Black=cells alone, gray=cells + secondary, orange=cells + isotype control antibody + secondary