Histone H4 Antibody / HIST1H4A

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000

UniProt

P62805

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

This Histone H4 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 71-103 amino acids from the C-terminal region of human HIST1H4A.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Histone H4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Histone H4 antibody is available for research use only.

Storage Conditions

Aliquot the Histone H4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

All species

Image Legend

Western blot analysis of 1) human HL60, 2) mouse NIH 3T3, 3) human HeLa, 4) human A431, 5) rat H-4-II-E and 6) rat C6 cell lysate using Histone H4 antibody at 1:2000.